A highly efficient polyethylene glycol-mediated transformation method for mushrooms
نویسندگان
چکیده
منابع مشابه
Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens
A simple and efficient method to transform Physcomitrella pantens protoplasts is described. This method is adapted from protocols for Physocmitrella protonemal protoplast and Arabidopsis mesophyll protoplast transformation. Due to its capacity to undergo efficient mitotic homologous recombination, Physcomitrella patens has emerged as an important model system in recent years. This capacity allo...
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We have established an efficient procedure for protoplast transformation and regeneration of fertile transgenic plants of rice (Oryza sativa L.) cultivars Nipponbare and Taipei 309. Protoplasts were mixed with a plant-expressible hygromycin resistance gene and treated with 25% (w/v) polyethylene glycol. Stringent selection of transformed colonies was applied to 14-day-old regenerated protoplast...
متن کاملA polyethylene glycol-mediated protoplast transformation system for production of fertile transgenic rice plants.
We have established an efficient procedure for protoplast transformation and regeneration of fertile transgenic plants of rice (Oryza sativa L.) cultivars Nipponbare and Taipei 309. Protoplasts were mixed with a plant-expressible hygromycin resistance gene and treated with 25% (w/v) polyethylene glycol. Stringent selection of transformed colonies was applied to 14-day-old regenerated protoplast...
متن کاملPolyethylene glycol precipitation as a screening method for macroprolactinemia.
To the Editor: Vieira et al. (1) used the Wallac Delfia immunofluorometric assay to demonstrate that macroprolactin is a common cause of apparent hyperprolactinemia, and this confirms our experience (2) and that of others (3). However, their data validating the polyethylene glycol (PEG) precipitation as a screening method for detecting macroprolactinemia are substantially different than ours (2...
متن کاملComparison between polyethylene glycol (PEG)- and polyethylenimine (PEI)-mediated transformation of Aspergillus nidulans Authors
Genetic transformation of many filamentous fungi is carried out by a protocol that utilizes polyethylene glycol (PEG) and calcium ion (Ca). This method has remained practically unchanged for more than 20 years, but the roles these molecules play are not definitively understood. To gain a better understanding, we have compared PEG transformation to a protocol using polyethylenimine (PEI) that is...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2006
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.2006.00110.x